Immunoassay Testing

Immunoassay Testing Updates & Advancements from Tosoh Bioscience

(April 26, 2009) … Part 1 … Immunoassay Testing Video Training focusses on Reagents and Samples on the Tosoh AIA-600 II Automated Immunoassay Analyzer.  

Reagents: There are three common reagents used on the 600 II.  All assays use the same wash, diluent, and substrate.  Sample diluting solutions, calibration/verification material, and calibrators are specific for each analyte.  Refer to the analyte application manual for information about any of our reagents.

Substrate II is packaged with 2 sets per box.  Each set consists of one bottle of lyophilized substrate and one 100 ml bottle of reconstituant.  Add the entire contents of the bottle of reconstituant to the bottle of substrate.  Once it has dissolved it is ready to use.  Once the substrate has been reconstituted it should be protected from light.  It is stable for 3 days if kept at room temperature and 7 days if kept in the refrigerator after use.

Do not mix one bottle of substrate with another.  Do not add new substrate to substrate that is about to expire.  Label with the date you reconstitute it so you will know when it will expire.

Diluent and Wash

Both are package with 4 100 ml bottles of concentrate per box.  In the concentrate form they are stored in the refrigerator and aare usable up to the expiration date.  Once they are reconstituted they are stable for 30 days.

Diluent is made by adding 800 ml of Type 1 Reagent Grade water to the 1 liter reagent reservoir, then add 20 ml of diluent concentrate and then filling the reservoir up to the 1 liter mark on the reservoir.  Wash is made the same way except that 40 ml of wash concentrate is used.  Label with the expiration date.

Both wash and diluent need to be aged for 24 hours before use.  The 600 II comes with 2 sets of reagent reservoirs so you can use one set to make up the wash and diluent  ahead of the time they are needed.

Test Cups
Test cups are packaged in two different sizes.  The 40 minute incubation tests are packed in boxes of 200.  Each containing 10 trays of 20 cups each.  The ST analytes are packaged in boxes of 100, containing 5 trays of 20 cups each.  The cups are stable up to the expiration date on the front of the box if they are stored in the refrigerator at 2 – 8 degrees C.  Refer to the analyte application manual for information about stability at room temperature.

For complete script from the AIA-600 II Immunoassay Testing Training Video,
go to Tosoh Media